Extended Data Figure 5 |. Primary antibody specificity.

a, Coomassie-stained CN-PAGE gel after transferring isolated mouse skeletal muscle mitochondrial proteins to PVDF membrane for western blotting. Mitochondrial oxidative phosphorylation complexes I–V can be resolved as individual bands. b, Western blot dual immunostained for complex V, β-subunit (upper band), and complex IV, subunit I (lower band), with the same primary antibodies as used for the muscle section dual immunostaining as shown in Fig. 3 and Extended Data Fig. 4.