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. Author manuscript; available in PMC: 2020 Jan 29.
Published in final edited form as: Biochem J. 2013 Apr 1;451(1):33–44. doi: 10.1042/BJ20121307

Table 2. Binding of PDBu and DOG to PKCθC1B domain variants.

WT, wild-type.

PKCθ C1B WT PKCθ C1B Y239A PKCθ C1B T243A PKCθ C1B W253G PKCθ C1B L255G PKCθ C1B Q258G
PDBu Kd (nM)* 0.39 ± 0.2 3.32 ± 0.34 0.64 ± 0.06 2.95 ± 0.19 4.44 ± 0.56 670 ± 150
DOG Ki (nM)* 16.1 ± 0.7 86 ± 15 21.5 ± 1.8 470 ± 69 76 ± 24 1840 ± 320
Ki/Kd 41 26 34 159 17 3
SAG Ki (nM)* 8.7 ± 2.0 35.1 ± 5.1 5.5 ± 1.5 271 ± 22 71 ± 10 2260 ± 330
Ki/Kd 22 11 9 92 16 3
*

Values represent the means ± S.E.M. of triplicate independent experiments.