Table 3. Quantification of membrane/cytoplasmic intensity ratios in confocal images.

Membrane/cytoplasmic intensity ratios were calculated from the confocal images of the multiple experiments. Values are the means ± S.E.M. of at least three images for 1000 nM and 100 nM PMA-treated samples and at least two images for the 10 nM PMA treated samples. WT, wild-type.

Treatment	Time (min)	PKCθ WT	PKCθ Y239A	PKCθ T243A	PKCθ W253G	PKCθ L255G	PKCθ Q258G
100 nM PMA	5	14.5 ± 2.2	3.8 ± 1.8	10.3 ± 1.9	11.4 ± 3.6	4.8 ± 1.5	1.2 ± 0.1
	10	11.3 ± 2.1	4.2 ± 1.6	9.9 ± 1.5	12.7 ± 3.9	6.0 ± 1.2	2.0 ± 0.3
	20	12.1 ± 2.2	5 ± 0.4	7.4 ± 1.5	13.2 ± 5.0	7.2 ± 1.9	2.1 ± 0.4
100 nM PMA	5	8.1 ± 1.2	1.7 ± 0.4	3.6 ± 1.1	1.9 ± 0.4	1.3 ± 0.1	1.0 ± 0.0
	10	6.5 ± 0.7	2.3 ± 0.8	3.7 ± 0.8	3.0 ± 0.7	1.6 ± 0.2	1.1 ± 0.0
	20	5.4 ± 0.5	2.3 ± 0.5	3.1 ± 0.6	2.6 ± 0.6	2.3 ± 0.4	1.1 ± 0.0
10 nM PMA	5	1.0 ± 0.1	1.1 ± 0.0	1.0 ± 0.0	1.0 ± 0.1	1.1 ± 0.1	1.2 ± 0.1
	10	1.0 ± 0.1	1.1 ± 0.1	1.3 ± 0.2	1.1 ± 0.1	1.1 ± 0.1	1.1 ± 0.1
	20	1.2 ± 0.4	1.0 ± 0.0	1.7 ± 0.1	1.2 ± 0.2	1.1 ± 0.0	1.1 ± 0.0