Fig. 4. Binding of 5heC by 5hmC ‘reader’ protein. (A) UHRF2 binds to 5hmC (and potentially to 5heC) and interacts with a range of proteins for enhanced chromatin accessibility. (B) Structure of 5heC phosphoramidite 5. (C) Fluorescence anisotropy measurement showing that UHRF2 binds double-stranded DNA carrying either 5hmC or 5heC with similar affinity.
