Fig. 6. Activity of TDG towards 5heC. (A) Schematic showing an assay involving oxidation of 5hmC (R = H) or 5heC (R = CH3) on FAM-labeled DNA, duplex formation and base excision by TDG. (B and C) TDG can excise 5hmC (B) and 5heC (C) only in the presence of KRuO4 as confirmed by in-gel fluorescence. S: substrate DNA; P: product DNA.
