Fig. 7. Selective labeling of 5heC. (A) Schematic showing a tandem oxidation and biotinylation approach to functionalize 5hmC and 5heC. (B) β-GT can transfer both glucose (R2 = OH) and N3-glucose (R2 = N3) to 5hmC (R1 = H) but only glucose to 5heC (R1 = CH3). (C) Dot-blot assay with anti-biotin antibody confirms tandem KRuO4 oxidation and oxime formation on 5hmC and 5heC. ARP: aldehyde reactive probe (Fig. S12† for structure).
