Figure 2.

Intracellular expression of antimicrobial peptides assessed by immunocytochemistry (ICC) and flow cytometry. A, Mesenchymal stem cells (MSC) were immunostained with antimicrobial peptide antibodies, as described in the Materials and Methods section. Positive binding of antibodies to intracellular peptides is depicted as red staining. Immunostaining with matched irrelevant isotype antibodies are shown in bottom right inset boxes. Images shown in ×20 magnification. B, Intracellular immunostaining of antimicrobial peptides in MSC, cathelicidin LL‐37, beta defensin (hBD2), hepcidin, surfactant protein D (SPD), and lipocalin (LcN) as assessed by flow cytometry. Mean fluorescence intensity on x‐axis. Intracellular immunostaining with irrelevant control isotype matched antibody (black dotted line), positive staining in red. Figures are representative of results obtained using three different donor MSC