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. 2019 Nov 26;7:362. doi: 10.3389/fbioe.2019.00362

Figure 2.

Figure 2

Function characterizations of displayed Fab D2E7. (A) Recombinant production of human TNFα as a His6 tagged protein. (B) Flow cytometry analysis of cells carrying the gene of Adalimumab (D2E7) heavy chain (VH-CH1) without its light chain. Expression was induced by 20 g/L galactose and cells were labeled with 20 nM TNFα-His6 and 0.1 μM anti-His6-iFluor647. (C) Flow cytometry analysis of cells carrying D2E7 Fab display construct. Induced cells were labeled with 1 pM −20 nM TNFα-His6 and 0.1 μM anti-His6-iFluor647 for TNFα+ cell quantification. (D) Sigmoidal curves depicting fluorescence intensities of TNFα+ cells as a function of TNFα concentrations. Data are presented as mean ± S.E. (n = 3 independent experiments). MFI, mean fluorescence intensity.