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. 2019 Nov 26;7:362. doi: 10.3389/fbioe.2019.00362

Figure 3.

Figure 3

Effects of molecular chaperones on Fab functional display. (A) Co-expression of Kar2p or Pdi1p was under the control of an additional GAL1 promoter on pESD-Fab-Kar2p/Pdi1p. 0.1 μM anti-FLAG-iFluor647, 0.1 μM anti-HA-FITC, and 0.1 nM TNFα/0.1 μM anti-His6-iFluor647 were used for labeling induced cells. Display of heavy chain (FLAG+, B), display of light chain (HA+, C), efficiencies of functional display (defined as the percentage ratios between TNFα+ cells and HA+ cells, D) were presented as mean ± S.E. (n = 3 independent experiments) with Student's t-test being performed, *P < 0.05.