Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 26;7:362. doi: 10.3389/fbioe.2019.00362

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Mei, Li, Wang, Lee, Iverson, Zhang, Ge and Yi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Effects of ER-retention sequence (ERS) on Infliximab Fab functional display. (A) ERSs were fused to the C-terminus of Infliximab light chain. 0.1 μM anti-FLAG-iFluor647, 0.1 μM anti-HA-FITC, and 0.1–1.0 nM TNFα/0.1 μM anti-His₆-iFluor647 were used for labeling induced cells. Display of heavy chain (FLAG⁺, B), display of light chain (HA⁺, C), efficiencies of functional display (TNFα⁺ cells/HA⁺ cells, D) were presented as mean ± S.E. (n=3 independent experiments) with Student's t-test being performed, *P < 0.05. Effects of four ERSs (KDEL, HDEL, FEHDEL, and WEHDEL) were tested and compared to the clones without ERS.