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. 2019 Nov 26;7:362. doi: 10.3389/fbioe.2019.00362

Figure 5.

Figure 5

FACS enrichment for high affinity cb2-6 Fab clone from (A) 1:103 and (B) 1:105 spiked libraries. Cells carrying cb2-6 Fab gene were mixed with cells carrying D2E7 Fab gene at an initial ratio of 1:103 or 1:105. After induction, mixed cells were incubated with TNFα-His6 of decreasing concentration over the rounds and 0.1 μM anti-His6-iFluor647. Sorting gates with their population percentages of representative rounds are shown. After each round of sorting, 10 clones were randomly picked for plasmid extraction and DNA sequencing. (C) TNFα-His6 concentrations and post-round proportion of cb2-6 clones for each sorting rounds. n.d., not determined.