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. 2018 Jul 23;33(1):469–483. doi: 10.1096/fj.201800436R

Figure 5.

Figure 5

The H2S donor GYY4137 improved urine concentration and prevented the reduction of AQP-2 protein levels in the inner medulla of kidneys from lithium-treated mice. A–C) Urine output (A), urine osmolality (B), and creatinine clearance (C) were examined on d 7 for lithium-treated mice with or without GYY4137 treatment. D, E) Representative semiquantitative AQP-2 immunoblots and the corresponding densitometric analyses of protein expression levels normalized to β-actin (n = 5 in controls and GYY groups; n = 7 in Li and Li+GYY groups). The experiment was repeated 3 times. CTL, control group; GYY4137, GYY4137 treatment group; Li, lithium treatment group; Li+GYY4137, lithium plus GYY4137 treatment group. A, B, E) *P < 0.05 vs. controls, #P < 0.05 vs. lithium-treated mice. F, G) GYY4137 increased AQP-2 protein expression in IMCD cell suspensions prepared from lithium-treated rats. *P < 0.05 vs. control-treated with vehicle, #P < 0.05 vs. lithium treatment.