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. 2019 Nov;33(11):12364–12373. doi: 10.1096/fj.201900815RR

Figure 3.

Figure 3

Validation of p16tdTom reporter. A) Gene expression for p16Ink4a and Arf for control and senescence-induced explants from 3 litters of mice. Data were normalized to TATA-binding protein housekeeping control and then to the mean of control samples. B) Western blot for p16Ink4a and Gapdh using protein from hip cartilage explants or MEFs. Three litters of p16tdTom/p16Ink4a heterozygous mice were used in control and senescence-inducing conditions. Mice that were homozygous for p16tdTom are p16Ink4a null but have intact Arf. MEFs labeled as p16Ink4a knockout had a global knockout of both p16Ink4a and Arf. C) Quantification of p16Ink4a protein band intensity normalized to Gapdh and then to the mean of control samples.