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Inhibition of PKA in LPS-stimulated RAW264.7 cells down-regulates the expression of iNOS and Cox2. Representative immunoblot (A) and quantitation of iNOS (B) and Cox2 (C) levels in NTC and Crth2−/− RAW264.7 cells treated with DMSO or H89 (30 µM) after 8 and 12 h of LPS stimulation (100 ng/ml) or no stimulation (0 h). β-Actin is used as loading control. *P < 0.05 compared with DMSO (Student’s t test; n = 3 independent experiments). VC, vehicle.
