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. 2019 Nov;33(11):12838–12852. doi: 10.1096/fj.201802608R

Figure 8.

Figure 8

TNF-α is up-regulated by the miR-155–Carhsp1 axis in Crth2−/− RAW264.7 cells. A) Mean fold change of mRNA levels of miR-155 in NTC and Crth2−/− RAW264.7 cells after 8 h of LPS stimulation (100 ng/ml); n = 6 independent experiments. B, C) Representative immunoblot (B) and densitometric quantitation (C) of Carhsp1 levels in NTC and Crth2−/− RAW264.7 cells after 8 h LPS stimulation (100 ng/ml). β-Actin is used as loading control; n = 4 independent experiments. D) Tnf-α production 1 h after LPS stimulation. n = 3 independent experiments; n = 3 independent experiments. E) Mean fold change of mRNA levels of Carhsp1 8 h after LPS stimulation relative to NTC RAW264.7 cells; n = 3 independent experiments. *P < 0.05, ****P < 0.0001 compared with NTC (Student’s t test).