Figure 6.

Fas DD–FADD interaction with sevoflurane. A) Predicted sevoflurane binding site on FADD–Fas DD complex. B) Using HEK cells transiently transfected with Fas WT-YFP and FADD WT-CFP, FRET experiment was performed with or without 1% isoflurane or 2% sevoflurane. Data were shown as means ± sd of triplicates. Two independent experiments were performed. Statistical analysis was performed using 1-way ANOVA with Bonferroni post hoc analysis; n.s., not significant. **P < 0.01. C) Nearby residues from docked sevoflurane site were shown. Amino acids from FADD are denoted with an asterisk.