Figure 4.

Cell–cell contact leads to expression of a high MW form of striatin. A) Immunoblot analysis of total cell lysates from MDCK, MCF7, Huh7, COS-7, and HEK293T grown to high confluency and reacted with the indicated antibodies. B) SW480 cells were seeded in a semiconfluent culture dish at 6 × 10³ cells/cm² (Sparse-S) and 6 × 10⁴ cells/cm² (Dense-D) and analyzed by Western blot for the expression of striatin, E-cadherin, and tubulin. C) HEK293T cells grown in sparse (S) or dense (D) cultures were separated into Triton X-100 soluble and insoluble fractions and were analyzed by Western blot to assess the expression levels of E-cadherin, striatin, and tubulin. D) MDCK cells grown as dense cultures were fractionated into Triton X-100 soluble and insoluble fractions, and equal amounts of lysate were analyzed by Western blot for striatin and tubulin levels. E) Huh7 cells were transfected with the indicated siRNAs (100 nM) for 72 h, and the lysates were analyzed by SDS-PAGE.