Figure 7.

Tankyrase interacts and PARylates striatin. A) Lysates from HEK293T cells transfected with FLAG-striatin or control FLAG vector were immunoprecipitated using anti-FLAG antibodies and analyzed by immunoblotting with the indicated antibodies. B) Huh7 cell lysates were immunoprecipitated using the anti-PAR antibody, or IgG control, and then probed with antristriatin and anti-PAR antibodies. C) Huh7 cells were subjected to IF assay as previously described, using the anti-PAR and antistriatin antibodies. Scale bar, 50 µM.