Figure 1.

HNtg mice are resistant to GC-induced bone growth impairment. A, B) Four-week-old male wild-type and HNtg (C57BL6 background) mice were treated with/without Dexa (2.5 mg/kg body weight/d) for 28 consecutive days (n = 3–6/group). At end point, tibia (A) and femur (B) bone length were measured by digital caliper. C) Representative images indicating the different zones in the tibia growth plate in each group. Images stained with Alcian blue. Original magnification, ×20. D–F) The total height of the growth plate (D) as well as the height of R+P zone (E) and hypertrophic H zone (F) were analyzed in histologic sections of tibia growth plates. All error bars indicate sd. For statistical analysis, Student’s t test or Mann-Whitney Rank Sum Test was performed. *P < 0.05, **P < 0.01.