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. 2019 Jan 18;33(4):4962–4974. doi: 10.1096/fj.201801741R

Figure 3.

Figure 3

Local (ex vivo) and systemic antiapoptotic effects of HN. A, B) Quantitative immunohistochemistry of HN expression (brown staining) with Alcian blue counter staining in the growth plate of wild-type and HNtg mice (C57BL6 background) treated with/without Dexa (2.5 mg/kg body weight/d) for 28 consecutive days. n = 3–6; scale bars, 50 µm; original magnification, ×40. C, D) Quantitative TUNEL analysis in growth plate of FVB mice treated with HNG (n = 5), fluorescent staining of apoptotic cells is shown in red and DAPI-staining of nuclei in blue. E, F) Quantitative TUNEL analysis in growth plate of HNtg animals (n = 3–6) treated with Dexa (2.5 mg/kg body weight), HNG (100 µg/kg body weight), or both, for 28 consecutive days. Apoptotic cells stained brown (DAB) and marked with white arrows. All error bars indicate sd. For statistical analysis, 1-way ANOVA was performed. Original magnification (C, E), ×20. *P < 0.05, ***P < 0.001.