Figure 2.

Alleviation of tko^25t developmental delay in a second UAS-tko⁺ line. Time to eclosion at 25°C (means ± SD, n ≥ 3 replicate vials for each cross), of flies of the indicated genotypes, using various drivers plus the UAS-tko⁺(1) transgene, shown previously to confer a modest rescue of developmental delay without any driver [6]. Controls were FM7 balancer flies with transgene but without driver, as shown. Horizontal lines denoted by asterisks (*, **, ***) indicated significant differences in pairwise comparisons of flies of a given sex and tko genotype, with and without actively driven tko⁺(Student’s t test, p < 0.05, 0.01, 0.001, respectively. The specificity of each driver was confirmed by parallel crosses in which it was used to direct the synthesis of nuclear- or membrane-localized GFP (see Figure S1). Note that we avoided the use of the TM3 balancer because we established that it conferred a developmental delay in conjunction with tko^25t, whereas the chromosome 2 balancer CyO did not (Fig. S2).