Figure 7.
RNAi-mediated knockdown of Men (malic enzyme is synthetically (semi)lethal with tko25t. (a) Men RNA levels measured by qRTPCR and (b) time to eclosion of flies of the indicated genotypes (i.e. with relevant Men RNAi construct or balancer as shown), means ± SD, n ≥ 3 vials from each cross. Eclosion timing is shown for the temperature at which flies were also cultured for qRTPCR (25°C or 29°C as shown). Eclosion timing at the other temperature was qualitatively similar, as shown in Fig. S4A. Horizontal bars denoted by asterisks (**) indicate significant differences, in pairwise comparisons of RNAi and balancer control flies of each given genotype and sex analyzed (Student’s t test, p < 0.001). (c) Proportion (%) of eclosing RNAi male progeny that also carried tko25t, as opposed to the FM7 balancer, from crosses of the general type: tko25t/FM7; daGAL4 x FM7/Y; RNAi. Means ± SD, n ≥ 3 vials from each cross. Asterisks (***) above the bars denotes significant deviation from expected frequency of 50% (chi-squared test with Yates’ continuity correction, p < 0.001). See also Fig. S4. For a summary diagram of the reactions targeted by RNAi and by various drugs see Figure 10.