Table 1.
Primer pairs and probes used in this study.
| Locus | Primer/probe | 5ʹ–3ʹ sequence |
|---|---|---|
| Ubx | Ubx fwd | cctgttatccaatccgttgc |
| Ubx rev | agcgctcaaaaacaatctgg | |
| probe | FAM-ccgactcaactcactcgactcggcc-BHQ-1 | |
| Act5C | Act5C fwd | ctggcattcaacattcacc |
| Act5C rev | cgatttaacaaattcaaggcg | |
| probe | FAM-ccgtgcggtcgcttagctcagcctc-BHQ-1 | |
| lacZ | lacZ fwd | ccaaaatcaccgccgtaagc |
| lacZ rev | aggcgtttcgtcagtatccc | |
| probe | FAM-cagcgactgatccacccagtcccagac-BHQ-1 | |
| H23 | H23 fwd | agttcaagcccgggtattct |
| H23 rev | ccaagttggccagttttgat | |
| probe | FAM-cacaatcacacataaacacaaat-BHQ-1 | |
| Antp | Antp fwd | tcggttcattcccagttctt |
| Antp rev | gcgaaagtgaacgagaaacc | |
| probe | FAM-cactctaacggtcccgcgaag-BHQ-1 | |
| abd-A | abd-A fwd | cgaatttccgcgtaattgat |
| abd-A rev | gtctcgggttgcgttgtatt | |
| probe | FAM-ccccaaacacacacatgcgac-BHQ-1 | |
| upd2 | upd2 fwd | ttttcgagcatttgtcatgc |
| upd2 rev | cggaagtcgtgaatcgaaat | |
| probe | FAM-ctctcgctctctcggccgcc-BHQ-1 | |
| eve | eve fwd | gttggtacgctgggaatgat |
| eve rev | gttgccgggacttaatttga | |
| probe | FAM-atgttttgcccaacgaaaccga-BHQ-1 |