Figure 3.

Effect of eRpL22-like knockout on testes development. a) Dissecting microscope images of gross morphological changes to adult testis development resulting from heat shock treatment of embryos at 24–36 hours post-embryo deposition ([HPED]; bottom left) and 48–60 HPED (bottom right). Heat shock 24–36 HPED results in severe defects in both testis and accessory gland growth. Heat shock 48–60 HPED results in overall normal reproductive system. (+, CKO; +, CKO; HS-FLP/+). Scale bar: 300 μm. T- testis, AG- accessory gland, SV- seminal vesicle. SV/T indicates the tissue in the labeled region is either malformed testis, seminal vesicle, or both tissues. b) eRpL22-like knockout was confirmed through PCR analysis of DNA extracted from heat-shock treated CKO; HS-FLP/+ testis tissue compared to untreated control tissue.