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. 2018 Nov 29;12(3-4):143–163. doi: 10.1080/19336934.2018.1549419

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Figure 4. — Assessment of fertility in paralogue knockdown and rescue genotypes determined by number of eclosed progeny. F1 male fertility was assessed by number of eclosed progeny per male when mated with a single wildtype female. Compared to all other genotypes, eRpL22-like-FLAG rescue males have a significant decrease in number of progeny per male. eRpL22-like paralogue knockdown was performed with bam-GAL4-VP16. eRpL22-like-FLAG overexpression was driven by Actin-GAL4. The eRpL22-like OE and eRpL22 OE rescues were driven with Actin-GAL4. FLAG (FL), knockdown (KD), overexpression (OE), and rescue are listed below genotypes. Error bars represents one standard deviation, * represents a p < 0.01 according to t-test, graph represents the mean of 10 males. The genotypes of males from each fertility cross are listed from left to right: Wildtype, bam>eRpL22-like.IR, Act>eRpL22-like-FLAG, Act>eRpL22-like-FLAG/eRpL22.IR, Act>FLAG-eRpL22/eRpL22.IR.