Table 1.
Overview of outcomes of RNAi or overexpression crosses described. Viability was assessed using longevity assays. Motile sperm was assessed using testes squashes from phase contrast imaging; fertility was assessed in a 1:1 female: male ratio as described in Methods. Effects of paralogue depletion or overexpression were determined by Western analysis and ImageJ quantification.
| Cross | Viable | Motile Sperm | Fertile | Effect on Paralogue |
|---|---|---|---|---|
| Act>eRpL22.IR | No | - | - | - |
| Act>eRpL22-like.IR | Yes (>30 days) | Yes | Yes | Increase eRpL22 |
| bam>eRpL22-like.IR | Yes (>30 days) | Yes | Yes | Increase eRpL22 |
| Act>eRpL22-like-FLAG | Yes (>30 days) | Yes | Yes | Decrease in 33kD eRpL22 |
| Act>eRpL22-like-FLAG/eRpL22.IR | Yes (~14 days) | Yes, Reduced | Yes, Reduced | Bodies: Decrease eRpL22, Increase eRpl22-like Testes: No significant change |
| Act>FLAG-eRpL22/eRpL22.IR | Yes (>30 days) | Yes | Yes | No significant change |
| bam>eRpL22-like-FLAG/eRpL22.IR | Yes (>30 days) | Yes | Yes | No significant change |