Table 1. Primers used in the current study.
Primers | Primer forward | Primer reverse | PCR type |
---|---|---|---|
HSP70 | 5'-AACCACTTCGTTCAGGAGT-3' | 5'-TTGTTGTCCTTGGTCATGGC-3' | RT-PCR |
HSP90 | 5'-CAGTTCGGTGTGGGTTTCTA-3' | 5'-TTGTAGAAGTCGCCGTACTCC-3' | RT-PCR |
T7-HSP70 | 5'-TAATACGACTCACTATAG AACCACTTCGTTCAGGAGT-3' | 5'-TAATACGACTCACTATAG TTGTTGTCCTTGGTCATGGC-3' | (dsRNA synthesis) |
T7-HSP90 | 5'-TAATACGACTCACTATAG CAGTTCGGTGTGGGTTTCTA-3' | 5'-TAATACGACTCACTATAG TTGTAGAAGTCGCCGTACTCC-3' | (dsRNA synthesis) |
T7-GFP | 5'-TAATACGACTCACTATAG GTGGAGAGGGTGAAGG-3' | 5'-TAATACGACTCACTATAG GGGCAGATTGTGTGGAC-3' | (dsRNA synthesis) |
re- HSP70 | 5'-AACCACTTCGTTCAGGAGT-3' | 5'-CTCCTCTGTCGCTCGGTAT-3' | RT-qPCR |
re- HSP90 | 5'-CAGTTCGGTGTGGGTTTCTA-3' | 5'-GAGGATGACAAGCCCAAGAT-3' | RT-qPCR |
RL32 | 5'-TGGCACCACACCTTCTAC-3' | 5'-CATGATCTGGGTCATCTTCT-3' | RT-qPCR |