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. 2020 Jan 29;15(1):e0228164. doi: 10.1371/journal.pone.0228164

Fig 1. Vλ-expressing OmniChickens demonstrate normal B cell populations in the periphery.

Fig 1

(A) Circulating lymphocytes were isolated from SynVL-D (left panel) and SynVL-E OmniChickens (right panel) and stained for Bu1, a chicken B cell marker, chIgM (μ-specific), chIgL (chCL specific), huIgL (huCL specific) and two different T cell markers. Additionally, a polyclonal antibody raised against the inserted human transgenes was used (huVL/VH). Populations were compared to age-matched wild-type chickens, and no difference in B and T cell populations were observed (Student’s T-test, p>0.05). Error bars represent the standard deviation from the mean. (B) Representative plots from SynVL-E OmniChickens show a distinct population of cells expressing surface Ig, and two human specific antibodies demonstrate expression of the human V at the cell surface. Data are representative of several independent experiments.