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. 2020 Jan 29;15(1):e0228164. doi: 10.1371/journal.pone.0228164

Fig 2. Evaluation of plasma IgY in Vλ OmniChickens compared to wild-type and Vκ OmniChickens.

Fig 2

(A) Plasma from 5 week old wild-type (n = 5), SynVK-CK/SynVH-C/DKO (n = 4), SynVK-CK/SynVH-SD/DKO (n = 4), SynVL-D/SynVH-C/DKO (n = 4) and SynVL-E/SynVH-C/DKO (n = 7) and SynVL-E/SynVH-SD/DKO (n = 8) was assessed by non-quantitative IgY sandwich ELISA. Plasma was first diluted 1:1000 in buffer, followed by 5-fold serial dilutions. The results shown are representative of three independent experiments. The standard deviation between birds within a given genotype is shown. (B) In a thermal shift assay recombinantly expressed wild-type and transgenic immunoglobulins show no difference in melting temperature. The graph shown is representative of three independent experiments.