Skip to main content
. 2020 Jan 29;15(1):e0228164. doi: 10.1371/journal.pone.0228164

Fig 3. Vλ OmniChickens show broad epitope coverage of progranulin (PGRN), a multi domain model antigen.

Fig 3

A cohort of Vλ OmniChickens was immunized with PGRN and antigen specific clones identified using the GEM assay. (A) Epitope binning using high-throughput array SPR demonstrates that SynVL-D recognizes six of 7.5 distinct domains (bar 1) while SynVL-E OmniChickens generated antibodies to all 7.5 domains (bars 2 and 3). (B) A network plot illustrates the relationships between antibodies within a given epitope bin. A cohort of previously described antibodies from Vκ OmniChickens (red) and a set of 20 well characterized “standards” used to identify each Grn domain (yellow) were also included in the array and are shown in the nodal plot. Use of these additional antibodies enabled the identification of a subdomain within GrnA (A’) and also demonstrates the broad epitope space covered by the different OmniChicken genotypes. Vλ-derived antibodies are shown in blue.