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. 2020 Jan 29;40(5):974–995. doi: 10.1523/JNEUROSCI.1010-19.2019

Table 1.

Statistical analysis for all quantifications

Figure N Statistical test F values main effect/T value p value main effect p value (post hoc)
1H, DG height Control: 6 One-way ANOVA F(2,12) = 16.508 p = 3.58 × 10−4 20 dpi versus Control: p = 2.54 × 10−4
20 dpi versus 90 dpi: p = 0.027
20 dpi: 7
90 dpi: 5
1H, GCL thickness Control: 6 One-way ANOVA F(2,12) = 28.8 p = 2.62 × 10−5 20 dpi versus Control: p = 3.84 × 10−5
20 dpi versus 90 dpi; p = 1.02 × 10−4
20 dpi: 7
90 dpi: 5
1H, MF thickness Control: 6 One-way ANOVA F(2,11) = 44.908 p = 5.11 × 10−6 20 dpi versus Control: p = 4.61 × 10−6
20 dpi versus 90 dpi: p = 1.92 × 10−4
20 dpi: 5
90 dpi: 3
1H, NeuN density Control: 6 One-way ANOVA F(2,12) = 20.67 p = 0.0002 20 dpi versus Control: p = 0.004
20 dpi versus 90 dpi: p = 0.0026
20 dpi: 7
90 dpi: 5
2B Control: 10 t test T(21) = 0.4254 p = 0.6747
20 dpi: 13
2F, active NSCs density Control: 5 t test T(9) = 5.800 p = 0.0007
20 dpi: 6
2F, active NSCs fraction Control: 5 t test T(9) = 5.542 p = 0.0015
20 dpi: 6
2F, total neuroblast density Control: 5 t test T(9) = 4.516 p = 0.0027
20 dpi: 6
2F, early neuroblasts density Control: 5 t test T(10) = 4.883 p = 0.0028
20 dpi: 7
2F, early neuroblasts fraction Control: 5 t test T(10) = 1.140 p = 0.837
20 dpi: 7
2H, newborn neuron density Control: 5 t test T(9) = 4.977 p = 0.0025
90 dpi: 6
2H, neuron fraction/total CldU Control: 5 t test T(9) = 3.208 p = 0.0125
90 dpi: 6
3C 1 month: Two-way ANOVA Treatment: F(1,158) = 169.19 Treatment: p = 9.26 × 10−27 Control versus VEGF:
Control: 11 Time: p = 1.82 × 10−15     1 month: p = 5.82 × 10−4
VEGF: 15 Time: F(5,158) = 20.067     2 months: p = 5.11 × 10−4
2 months: Interaction: p = 1.339 × 10−4     3 months: p = 0.008
Control: 11 Time × treatment interaction (control/VEGF) × time: F(5,158) = 5.384     4 months: p = 9.48 × 10−9
VEGF: 15     5 months: p = 1.74 × 10−6
3 months:     7 months: p = 3.17 × 10−7
Control: 14
VEGF: 13
4 months:
Control: 13
VEGF: 15
5 months:
Control: 15
VEGF: 18
7m:
Control: 11
VEGF: 11
3D Control: 60 One-way ANOVA F(6,225) = 88.892 p = 1.39 × 10−6 Control versus 2 months: p = 4.2 × 10−06
1 month: 61 Control versus 3 months: p = 1.45 × 10−22
2 months: 15
3 months: 46 Control versus 4 months: p = 1.53 × 10−19
4 months: 16 Control versus 5 months: p = 8.32 × 10−18
5 months: 18
7 months: 11 Control versus 7 months: p = 5.75 × 10−34
4B Control: 13 t test CA1: T(21) = −10.752 CA1: p = 9.21 × 10−10
VEGF: 10 DG: T(21) = −8.237 DG: p = 7.39 × 10−8
4C Control: 12 t test CA1 neuron density: T(20) = −0.047 CA1 neuron density: p = 0.7
VEGF: 10 CA1 height: T(20) = −3.63 CA1 height: p = 0.002
5C No transgene: 4 One-way ANOVA GCL thickness: F(2,12) = 0.6605 GCL thickness: p = 0.5378
tTa only: 4 DG height: F(2,12) = 1.906 DG height: p = 0.199
tTa-tetGFP: 5
6D, NeuN density Control: 8 One-way ANOVA F(2,21) = 61.526 p = 1.655 × 10−9 Control versus VEGF On: p = 6.279 × 10−9
VEGF on: 8 Control versus VEGF On>off: p = 2.11 × 10−6
VEGF on>off: 8 VEGF On versus VEGF On>off: p = 0.002
6D, GCL thickness Control: 18 One-way ANOVA F(2,40) = 23.589 p = 1.7 × 10−7 Control versus VEGF On: p = 6.279 × 10−9
VEGF on: 9 Control versus VEGF On>off: p = 2.11 × 10−6
VEGF on>off: 16 VEGF on versus VEGF On>off: p = 0.002
6D, DG height Control: 18 One-way ANOVA F(2,40) = 30.116 p = 1.04 × 10−8 Control versus VEGF on: p = 1.15 × 10−8
VEGF on: 9 Control versus VEGF on>off: p = 0.01
VEGF on>off: 16 VEGF on versus VEGF on>off: p = 4.76 × 10−4
6D, MF thickness Control: 9 One-way ANOVA F(2,19) = 28.35 p = 1.9 × 10−6 Control versus On: p = 1.18 × 10−6
VEGF on: 9 Control versus on>off: p = 0.014
VEGF on>off: 7 On versus On>off: p = 0.01
6E Same as 3D Two-way ANOVA Treatment: F(2,161) = 207.169 Treatment: p = 2.99 × 10−45 Control versus 3 months on >1 month off: 4.83 × 10−13
On>off 1 month: 12 Control versus 3 months on >3 months off: p = 1.65 × 10−4
On>off 2 months: 16 Time: F(2,161) = 11.382 Time: p = 2.37 × 10−5 5 months on versus 3 months on >2 months off: p = 4.83 × 10−13
On>off 3 months: 30 7 months on versus 3 months on >3 months off: p = 4.82 × 10−13
Interaction: F(2,161) = 27.373 Interaction: p = 5.84 × 10−11
7D, total NSC density Control: 11 One-way ANOVA F(2,28) = 27.632 p = 0.22 Control versus VEGF On: p = 0.019
VEGF on: 7
VEGF on>off: 13
7D, active NSC density Control: 11 One-way ANOVA F(2,28) = 13.39 p = 0.0002 Control versus VEGF On: p = 0.0002 Control versus VEGF On>off: p = 0.0002
VEGF on: 7
VEGF on>off: 13
7D, fraction of active NSC Control: 11 One-way ANOVA F(2,28) = 4.379 p = 2.26 × 10−7 Control versus VEGF On: p = 4.24 × 10−7
VEGF on: 7 Control versus VEGF On Off: p = 2.03 × 10−5
VEGF on>off: 13
8B Control: 15 One-way ANOVA F(2,31) = 50.405 p = 1.8 × 10−10 Control versus VEGF On>off p = 5.47 × 10−9
VEGF on: 7 VEGF on versus VEGF On>off: p = 9.53 × 10−8
VEGF on>off: 12
8C, MVD Control: 19 One-way ANOVA F(2,34) = 154.153 p = 1.84 × 10−9 Control versus VEGF On: p = 3.33 × 10−7
VEGF on: 8 Control versus VEGF on>off: p = 3.47 × 10−8
VEGF on>off: 10
8C, total neuroblast density Control: 19 One-way ANOVA F(2,32) = 58.735 p = 1.9 × 10−11 Control versus VEGF On: p = 5.208 × 10−9
Control versus VEGF on>off: p = 2.12 × 10−8
VEGF on: 7
VEGF on>off: 10
8D, late neuroblast density Control: 14 One-way ANOVA F(2,28) = 26.836 p = 3.09 × 10−7 Control versus VEGF on p = 1.59 × 10−6
VEGF on: 6 Control versus VEGF on>off p = 1.69 × 10−5
VEGF on>off: 11
8D, late neuroblast % Control: 14 One-way ANOVA F(2,28) = 6.084 p = 0.006 Control versus VEGF on>off p = 0.01
VEGF on: 6 VEGF on versus VEGF on>off p = 0.031
VEGF on>off: 11
9D Control: 8 One-way ANOVA F(2,21) = 6.808 p = 0.005 Control versus VEGF On: p = 0.004
VEGF on: 7 VEGF On>off versus VEGF on: p = 0.028
VEGF on>off: 9
9F Control: 15 Two-way ANOVA Treatment: F(1,64) = 10.785 Treatment: p = 0.002 Control versus VEGF on: p = 0.004
VEGF: 9 Time: F(2,64) = 10.987 Time: p = 7.9 × 10−5
Time × treatment interaction: F(2,64) = 4.198 Interaction: p = 0.019
10B Control: 18 One-way ANOVA F(2,45) = 47.84 p = 7.27 × 10−12 Control versus VEGF On: p = 8.08 × 10−9
VEGF on: 15 Control versus VEGF On>off: p = 5.15 × 10−9
VEGF on>off: 15
10C, 12 months Control: 7 One-way ANOVA F(2,29) = 34.5 p = 1.55 × 10−5 Control versus VEGF On: p = 1.02 × 10−5; control versus VEGF On>off: 0.005
VEGF on: 4 VEGF On versus VEGF On>off: p = 0.003
VEGF on>off: 10
10C, 18 months Control: 10 One-way ANOVA F(2,21) = 11.07 p = 0.001 Control versus VEGF On: p = 3.93 × 10−4 Control versus VEGF On>off: p = 0.048
VEGF on: 5
VEGF on>off: 9
10C 12 months versus 18 months Two-way ANOVA Treatment: F(2,39) = 53.71 Treatment: p = 1.5 × 10−9 VEGF On 12 months versus VEGF on 18 months: p = 4.4 × 10−5
Age: F(1,39) = 31.749 Age: p = 1.66 × 10−6 VEGF On>off 12 months versus VEGF on>off 18 months: p = 0.015
Interaction: F(2,39) = 9.031 interaction: p = 0.001
10D 12 months: Two-way ANOVA Treatment: F(1,25) = 25.164 Treatment: p = 3.57 × 10−5 12 months Control versus On>off: p = 2.31 × 10−4 12 months On>off versus 18 months On>off: p = 3.83 × 10−4
Control: 6 Age: F(1,25) = 15.171 Age: p = 0.001
VEGF on>off: 5
18 months: Interaction: F(1,25) = 7.44 Interaction: p = 0.011
Control: 7
VEGF on>off: 11
10E, 12 months Control: 6 One-way ANOVA F(2,18) = 8.338 p = 0.003 Control versus VEGF On: p = 0.04
VEGF on: 4 VEGF On versus VEGF On-off: p = 0.04
VEGF on>off: 11
10E, 18 months Control: 9 One-way ANOVA F(2,17) = 20.527 p = 2.9 × 10−5 Control versus VEGF On: p = 1.37 × 10−5 Control versus VEGF On-off: p = 1.85 × 10−4
VEGF on: 5
VEGF on>off: 6
10E 12 months versus 18 months Two-way ANOVA Treatment: F(2,53) = 34.516 Treatment: p = 2.25 × 10−10 On>off 12 months versus On>off 18 months: p = 0.0148
Age: F(1,53) = 3.672 Age: p = 0.032
Interaction: F(2,53) = 3.166 interaction: p = 0.021
10F, 12 months Control: 8 One-way ANOVA F(2,21) = 7.61 p = 0.003 Control versus VEGF On: p = 0.002
VEGF on: 5
VEGF on>off: 11
10F, 18 months Control: 11 One-way ANOVA F(2,29) = 46.408 p = 9.16 × 10−10 Control versus VEGF On: p = 6.43 × 10−9 Control versus VEGF On>off: p = 3.06 × 10−7
VEGF on: 12
VEGF on>off: 9
10F 12 months versus 18 months Two-way ANOVA Treatment: F(2,50) = 36.843 Treatment: p = 1.466 × 10−10 On>off 12 months versus On>off 18: p = 0.025
Age: F(1,50) = 4.529 Age: p = 0.038
Interaction: F(2,50) = 3.192 interaction: p = 0.05
10G, 12 months Control: 8 One-way ANOVA F(2,21) = 8.175 p = 0.002 Control versus VEGF On: p = 0.003
VEGF on: 5 VEGF On versus VEGF On>off: p = 0.007
VEGF on>off: 11
10G, 18 months Control: 11 One-way ANOVA F(2,29) = 34.5 p = 2.14 × 10−8 Control versus VEGF On: p = 2.36 × 10−8
Control versus VEGF On>off: p = 1.62 × 10−5
VEGF on: 12
VEGF on>off: 9
10G 12 months versus 18 months Two-way ANOVA Treatment: F(2,50) = 30.784 Treatment: p = 1.93 × 10−9 On>off 12 months versus On>off 18 months: p = 0.0035
Age F(1,50) = 7.067 Age: p = 0.011
Interaction: F(2,50) = 4.568 interaction: p = 0.015