Figure 2.

Enhanced neurogenesis following overexpression of CX3CL1. A, P21 mice were BrdU pulse labeled for 24 h, and fixed brain sections were labeled by antibody to BrdU. The number of BrdU⁺ cells was significantly larger in Tg-CX3CL1 SGZ compared with that in wild-type controls. B, Stereological quantification confirmed greater numbers of BrdU⁺ dividing cells in P21 Tg-CX3CL1 SGZ. N = 5 pairs of mice (**p < 0.01, Student's t test). C, Mice at P45 were pulse labeled by BrdU and examined by confocal staining with antibody to BrdU (red) and NeuN (green) for mature neurons. D, BrdU⁺ cells from P45 mice were counted on 1 of every 10 continuous sections, and the number reflected an average per section. N = 5 pairs of mice (*p < 0.05). E, F, Eight-month-old mice were similarly BrdU pulse labeled and examined by immunohistochemical staining with antibody to BrdU. Visibly more BrdU⁺ cells were found in both Tg-CX3CL1 SGZ (E) and SVZ (F). Scale bar, 30 μm.