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. Author manuscript; available in PMC: 2020 Jul 13.
Published in final edited form as: Nat Chem Biol. 2020 Jan 13;16(2):206–213. doi: 10.1038/s41589-019-0447-7

Figure 3. LDAH converts LasA to LasF by cleaving its side chain.

Figure 3.

(a) LDAH may catalyze the hydrolysis of LasA to LasF by cleaving the ester bond (marked 2) in the side chain. (b) The abundances of LasA and LasF, normalized using the genomic DNA content in each sample, were measured by quantitative mass spectrometry (see Methods). (c) The ratio of the abundance of LasA to LasF for each independent experiment is depicted (on a logarithmic scale) for the four indicated cell lines. (d, e) Sensitivity to LasA and LasF in wildtype (WT) and LDAHnull cells was assessed using an MTT assay. IC50 values for LasA (d) in WT and LDAHnull cell lines are 19 ± 1 nM and 586 ± 34 nM respectively and for LasF (e) are 2.4 ± 0.1 μM and 4.3 ± 0.4 μM respectively. Circles denote the mean value (+/- S.D. from n=3 independent samples). Significance in b and c was tested using a two-tailed, unpaired t-test and is indicated as *** (p<0.001) or ** (p<0.01). The circles in b and c denote data from four different experiments in each of the indicated cell lines.