Figure 2:

Approaches to lasso peptide genome mining. Left: the enzyme-centric approach pioneered by Marahiel and co-workers. Putative B and C enzymes are identified first followed by searches for short ORFs nearby these enzymes. Subsequently developed tools such as PRISM and RODEO also use this approach. Right: the precursor-centric approach developed by Link and co-workers. Putative precursors are identified first via pattern matching followed by scoring of clusters based on the presence of conserved motifs within the B and C enzymes (asterisks).