TABLE 2.

Comparison of PCR C_T values for M. genitalium detection when the IC of the evaluated kit was added to the specimen (before DNA extraction) alone versus in addition to the other two ICs^a

Serial dilution of M. genitalium G37 culture (genome equivalent/μl)	Mean CT ± SD
	S-DiaMGRes (Diagenode)			RealAccurate TVMGres (Pathofinder)			ResistancePlus MG (SpeeDx)
	Diagenode IC alone	Three ICs	ΔCT	Pathofinder IC alone	Three ICs	ΔCT	SpeeDx IC alone	Three ICs	ΔCT
7.2 × 104	20.80 ± 0.21	21.43 ± 0.04	+0.63	20.71 ± 0.32	21.73 ± 0.39	+1.02	13.01 ± 0.00	12.96 ± 0.01	−0.05
1.2 × 103	26.48 ± 0.26	25.86 ± 0.20	−0.62	24.64 ± 0.40	24.81 ± 0.85	+0.17	17.02 ± 0.01	16.54 ± 0.14	−0.48
3.0 × 102	28.94 ± 0.52	28.00 ± 0.27	−0.94	27.29 ± 0.12	27.83 ± 0.17	+0.54	22.04 ± 0.02	21.98 ± 0.02	−0.07
2.8 × 101	34.38 ± 0.35	35.16 ± 0.55	+0.78	33.75 ± 0.04	34.44 ± 0.30	+0.69	26.47 ± 0.42	26.89 ± 0.54	+0.42

^aExperiments were performed in triplicate.