TABLE 5.
Handling, testing duration, and cost of the three evaluated commercial kits
| Commercial kit | No. of reagents per reaction mixture | Internal control | Thermal-cycling duration (min) | Positive and negative controls | Data analysis | List price/reaction excluding. taxes (€) |
|---|---|---|---|---|---|---|
| S-DiaMGRes Diagenode | 4 | Includeda | 1 h 45 min | Included | LightCycler 480 software (Fit point analysisd ) | 30 |
| Real Accurate TVMGres Pathofinder | 2 | Includedb | 1 h 35 min | Included | LightCycler 480 software (Abs Quant/2nd Derivative Maxd ) | 15 |
| ResistancePlus MG SpeeDx | 4 | Includedb | 1 h 25 min | Not includedc | UgenTec FastFinder ResistancePlus MG | 22 |
a
Inhibition control.
b
Extraction and inhibition control.
c
ResistancePlus MG positive-control kit is optional.
d
In accordance with the manufacturer’s instructions.