FIG 1.

Design of species-specific gRNA probes based on rpoB sequences. rpoB sequences were downloaded from M. tuberculosis H37Rv, M. abscessus (ATCC 19977), M. intracellulare (ATCC 13950), M. avium (ATCC 25291), M. kansasii (ATCC 12478), M. gordonae (ATCC 14470), and M. fortuitum (ATCC 6841). Multiple sequence alignment was performed using DNAMAN 8 software. Conserved sequences are blank. Red boxes represent gRNA probe targeted regions. Green boxes represent the PAM motif. Universal primers used for amplification of rpoB fragments are indicated by arrows.