FIG 2.

Illustration of FnCas12a/gRNA-based platform for identification of Mycobacterium species. rpoB fragments were specifically amplified from extracted DNA of bacteria by PCR. Species-specific gRNA probes were designed to target rpoB fragments. Unpurified PCR product was directly mixed with FnCas12a/gRNA/ssDNA-FQ (quenched fluorescent single-strand DNA reporter). Once gRNA probes match the target DNA and form a complex, FnCas12a will transcleave the quenched fluorescent ssDNA reporter, illuminating the fluorescence.