Determination of the specificity of the designed gRNA probes using reference Mycobacterium species. Bars 1 to 7 represent M. tuberculosis H37Rv, M. abscessus (ATCC 19977), M. intracellulare (ATCC 13950), M. avium (ATCC 25291), M. kansasii (ATCC 12478), M. gordonae (ATCC 14470), and M. fortuitum (ATCC 6841), respectively. Bars 8 to 12 represent E. coli, P. aeruginosa, A. baumannii, S. enteritidis, and K. pneumoniae, respectively. Error bars represent mean ± standard error of the mean (SEM), with n = 3 technical replicates.