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Evaluation of LOD with purified DNA and quantified CFU. (A) DNA extracted from pure- cultured reference strains were serially diluted in the range of 10 ng to 10 fg. (B) Serial quantified bacilli were spiked into artificial sputum. E. coli was used as negative control (NC). Error bars represent mean ± SEM, with n = 3 technical replicates.
