FIG 7.

Model of the regulatory network of direct regulators that regulate the expression of L. pneumophila EEGs. The four TCSs, CpxRA, PmrAB, LciRS, and LetAS, as well as the components of the LetAS-RsmYZ-CsrA regulatory cascade, which were found to be involved in the regulation of EEGs, are shown. The four SHKs (CpxA, LetS, PmrB, and LciS) are drawn inside the bacterial inner membrane. The four RRs (CpxR, LetA, PmrA, and LciR), as well as the other DNA binding regulators (Fur, Fis1, and Fis3), are colored purple. The RRs are phosphorylated (small yellow circle) and/or dephosphorylated by their cognate SHKs. The CsrA RNA binding regulator is colored green, and the RsmY/RsmZ small RNAs are colored blue. Connector proteins that participate in the regulation of EEGs are colored red, and modulators are colored white. Acetyl-P, acetyl phosphate; PEP, phosphoenolpyruvate; PTS, phosphotransferase system. The numbers of EEGs that were shown to be regulated by each of the regulatory systems are indicated in parentheses. Arrows and T-shaped symbols indicate activation and repression, respectively. Solid lines indicate direct regulation and broken lines indirect regulation.