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. 2020 Jan 29;6(5):eaay2756. doi: 10.1126/sciadv.aay2756

Fig. 5. The osteogenic differentiation and stemness maintenance of hBM-MSCs on the molecular motor surfaces.

Fig. 5

(A) Fluorescent images of hBM-MSCs cultured on molecular motor surfaces with different cell media for 14 days. Scale bars, 50 μm. Red, OPN; blue, nucleus. (B) Protein level: Quantification of the expression of OPN normalized by the cell number over 14 days of culture (n = 3). (C) Gene level: Real-time PCR (RT-PCR) analysis of relative mRNA expression levels of OPN genes in different media over 14 days of culture (n = 3). (C and D) RT-PCR analysis of relative mRNA expression level of Nanog and OCT4 genes at different time points (n = 3; *P < 0.05).