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. Author manuscript; available in PMC: 2020 Jan 29.
Published in final edited form as: J Vis Exp. 2019 Feb 13;(144):10.3791/59266. doi: 10.3791/59266

Figure 3: Comparison of IF images of blood vessels acquiredwith or without optical clearing step with 90% glycerol.

Figure 3:

(A) Whole-mount immunofluorescence (IF) staining with anti-endomucin antibody (green) in sWAT. The sample was not subjected to the optical clearing step (step 3.10). (B) Whole-mount IF staining with anti-endomucin antibody in sWAT. The sample was subjected to the optical clearing step (step 3.10) before the mounting steps (steps 3.11 and 3.12).