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Microscopic examination of sclerotia. Sclerotia production in A. flavus SRRC1582 wild-type (WT) and a ∆pks27 mutant was observed using an Olympus SZH10 stereomicroscope (x96 magnification) and Nikon DS-Qi1 camera. Strains were grown on GMMS agar for 10 days in the dark at 30°C. Note loss of dark pigmentation in sclerotia of the ∆pks27 mutant due to abrogation of production of asparasone A.
