. Author manuscript; available in PMC: 2020 Jan 29.

Published in final edited form as: Nature. 2018 Jul 25;560(7716):122–127. doi: 10.1038/s41586-018-0362-1

Extended Data Table 2. Mutant mouse REV7 proteins generated and phenotyped in this study.

Name	REV7 mutants	Description	References
Safety belt	REV7^Y63A REV7^W171A REV7^Y63A,W171A	Predicted to block REV7-REV3L interactions and sensitizes DT40 cells to Cisplatin	17
Mouse mutant alleles	REV7^C70R	Mouse missense mutation predicted to disrupt DNA polymerase ζ formation; sensitizes cells to ICLs.	31
Rev1-binding residues	REV7^L186A REV7^Q200A REV7^Y202A REV7^L186A,Q200A	Predicted to block REV7-REV1 interactions	16,32
PTM/Degron	REV7^R6A,L9A	D-box mutation blocks APC/C-dependent Rev7 degradation	18
	REV7^{K44R, K90R, K162R, K167R, K190R}	Identified REV7 ubiquitinated sites	33 Phosphosite.org
	REV7^{K44R, K46R, K47R, K72R, K77R, K82R, K90R, K97R, K129R, K162R, K167R, K190R, K198R, K208R}	Lysine-less REV7 mutant
	REV7^T191A REV7^T191E	Reported phosphorylation site.	Phosphosite.org
Conserved surface residues	REV7^K129A REV7^K129R	Conserved Lysine	Identified by structure-led conservation analysis
Conserved surface residues	REV7^D17A REV7^D17R REV7^E21A REV7^E21R REV7^E24A REV7^E24R REV7^E120A REV7^E120R	Conserved acidic patch	Identified by structure-led conservation analysis