Extended Data Table 3. CRISPR-Cas9 reagents and edited cell-lines generated in this study. (A) Sequences of sgRNA used in gene editing experiments. S, indicates sgRNA corresponds to sense strand; AS, indicates antisense strand. (B) Individual edited alleles in each cell-line clone as confirmed by Sanger sequencing.
| a | ||||
|---|---|---|---|---|
| Target gene name / sgRNA # | Sequence (5’-3’) | Description | ||
| Rev7 (Mad2l2/Mad2b) | CCTGATTCTCTATGTGCGCG | Targeted to exon 4, S | ||
| C20orf196 / Shld1 | 1 | CTGTACCTTGGATCTACCCG | Targeted to exon 2, S | |
| 2 | TCTTCTTCGACTACTGATCC | Targeted to exon 2, S | ||
| Fam35a / Shld2 | 1 | TCGGACTTTTTCCCAGGCGT | Targeted to exon 4, AS | |
| 2 | CAGGGAGTCAGACGAACTGA | Targeted to exon 4, AS | ||
| 3 | ACTGCATGGACTAATATATC | Targeted to exon 6, AS | ||
| Flj26957 / Shld3 | 1 | GACTCATCGTATGGAAACCA | Targeted to exon 1, AS | |
| 2 | GGAAGTTTGGACTCATCGTA | Targeted to exon 1, AS | ||
| b | ||||
| CH12-F3 | Target gene | Clone | Genotype | |
| Allele 1 | Allele 2 | |||
| Rev7 | C | +1 bp at R34 | -2 bp at R34 | |
| C20orf196 / Shld1 | B2 | +2 bp at L20 | +2 bp at L20 | |
| C1 | -14 bp at S54 | +1 bp at D58 | ||
| D1 | -5 bp at T57 | -5 bp at T57 | ||
| Fam35a / Shld3 | E4 | -1 bp at A35 | +32 bp at A35 | |
| F7 | -25 bp at T32 | -19 bp at N34 | ||
| G11 | -5 bp at R273 | -20 bp at G270 | ||
| Flj26957 / Shld3 | A2 | -1 bp at W46 | -1 bp at W46 | |
| A4 | - 2 bp at F42 | -7 bp at I44 | ||
| F9 | -2 bp at Y49 | -2 bp at Y49 | ||
| H3 | -4 bp at Y49 | -13 bp at K53 | ||
| Flj26957 in Rev7-/- (C) | A2 | -23 bp at F38 | -23 bp at F38 | |
| C7 | -22 bp at F38 | -22 bp at F38 | ||
| KB1P-G3 (Brca1-/- p53-/-) | Flj26957 | 7 | -1 bp at W46 | -8 bp at W46 |
| 8 | -1 bp at W46 | -5 bp at W46 | ||