Extended Data Table 4. Primers used in this study. (A) Primers used to amplify CRISPR-Cas9 target loci in edited cell-lines. (B) Locus-specific ChIP amplicon qPCR primer sequences.

a
Target/gene locus		Primer Sequence (5’-3’)
Rpp30	Fwd	TCCAGTGTGCAAGAAAGCTAAATG
	Rev	GGCAGTGCGTGGAGACTCA
A (Igh S µ)	Fwd	CAATGTGGTTTAATGAATTTGAAGTTGCCA
	Rev	TCTCACACTCACCTTGGATCTAAGCACTGT
B (Igh S µ)	Fwd	GCTAAACTGAGGTGATTACTCTGAGGTAAG
	Rev	GTTTAGCTTAGCGGCCCAGCTCATTCCAGT
C (Igh Sγ1)	Fwd	AGTGTGGGAACCCAGTCAAA
	Rev	GTACTCTCACCGGGATCAGC
D (Igh S α)	Fwd	TGAAAAGACTTTGGATGAAATGTGAACCAA
	Rev	GATACTAGGTTGCATGGCTCCATTCACACA

b
Target gene	sgRNA	Sequence (5’-3’)
C20orf196 / Shld1	1	Fwd	AGTAGCTGCTCTTTTGGCGT
		Rev	TGGCAGCAGAAAGAGAAGGG
	2	Fwd	AGTAGCTGCTCTTTTGGCGT
		Rev	TGGCAGCAGAAAGAGAAGGG
Fam35a / Shld3	1	Fwd	AGCCCACACATTTGTCCACT
		Rev	GCTCCAGTTGCTCCACTGAA
	2	Fwd	TCCTTGGCTTCTTGGACACC
		Rev	ATGGAGGCAGAACCAACAGG
	3	Fwd	TGACCTTGAGCCTGTTCCAC
		Rev	TGGAGTTGGAGCAGTTGCAG
Flj26957 / Shld3	1	Fwd	TACTGCTTCACGCTCTCAGC
		Rev	GCAGGCTGTCCCTACCAAAT
	2	Fwd	TACTGCTTCACGCTCTCAGC
		Rev	GCAGGCTGTCCCTACCAAAT