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. Author manuscript; available in PMC: 2020 Dec 11.
Published in final edited form as: Cell Host Microbe. 2019 Nov 26;26(6):795–809.e5. doi: 10.1016/j.chom.2019.10.007

Figure 7: S. aureus alpha toxin limits accumulation of antigen-specific Tregs.

Figure 7:

(A) Neonatal mice colonized with S. aureus-2w or S. aureus-2w-ΔagrA, S. aureus-2w-Δhla or S. aureus-2w-Δpsm on postnatal day 7, 10 and 13 and then re-challenged 2 weeks later with intradermal 2w peptide in Incomplete Freund’s Adjuvant (IFA). Representative flow cytometry plots and percentages of 2w-specific Tregs in the SDLNs 8 days later. (B) Neonatal mice were colonized as above with either S. aureus-2w, S. aureus-2w-Δhla or S. aureus-2w-Δpsm and then re-challenged 2 weeks later with intradermal 2w peptide in IFA. (C) Neonatal mice were colonized with S. epi as above with or without recombinant hla and challenged 2 weeks later with intradermal 2w peptide in IFA. Representative flow cytometry and percentages of 2w-specific Tregs 8 days later. All plots are gated on live 2w+DUMPnegTCRb+CD4+CD44+ cells in the tetramer-enriched fraction. Data are representative of two independent experiments with at least 3 mice per group. See also Figure S7.