Key Resources Table
| Reagent | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Per-Cp-Cy5.5 anti-mouse TCR-beta (clone H57-597) | Biolegend | Cat# 109228; RRID: AB_1575173 |
| Per-Cp-Cy5.5 anti-mouse Ly6G (clone 1A8) | Biolegend | Cat# 127616; RRID: AB_1877271 |
| Per-Cp-Cy5.5 anti-mouse CD24 (clone M1/69) | Biolegend | Cat# 101823; RRID: AB_1595596 |
| FITC anti-mouse/Rat ICOS (clone C398.4A) | Thermo Fisher Scientific | Cat# 11-9949-82; RRID: AB_465458 |
| FITC anti-mouse CD3 (clone 17A2) | BD Biosciences | Cat# 555274; RRID: AB_395698 |
| FITC anti-mouse GL3 (clone eBioGL3) | Thermo Fisher Scientific | Cat# 11-5711-82; RRID: AB_465238 |
| Pe-Cy7 anti-mouse CD8 (clone 53-6.7) | BD Biosciences | Cat# 552877; RRID: AB_394506 |
| Pe-Cy7 anti-mouse CD11c (clone HL3) | BD Biosciences | Cat# 558079; RRID: AB_647251 |
| Pe-Cy7 anti – mouse pro-IL-1 beta (NJTEN3) | Thermo Fisher Scientific | Cat# 25-7114-82; RRID: AB_2573526 |
| PE anti-mouse CD4 (clone GK1.5) | Biolegend | Cat# 100407; RRID: AB_312692 |
| PE anti-mouse CD207 (clone eBioL31) | Thermo Fisher Scientific | Cat# 12-2075-82; RRID: AB_763452 |
| APC-efluor 780 anti-mouse B220 (clone RA3-6B2) | Thermo Fisher Scientific | Cat# 47-0452-80; RRID: AB_1518811 |
| APC-efluor 780 anti-mouse CD3 (clone 145-2C11) | Thermo Fisher Scientific | Cat# 47-0031-82; RRID: AB_11149861 |
| APC-efluor 780 anti-mouse CD11b (clone M1/70) | Thermo Fisher Scientific | Cat# 47-0112-82; RRID: AB_1603193 |
| APC-efluor 780 anti-mouse CD11c (clone N4818) | Thermo Fisher Scientific | Cat# 47-0114-82; RRID: AB_1548652 |
| APC-efluor 780 anti-mouse F4/80 (clone BM8) | Invitrogen | Cat# 47-4801-82; RRID: AB_2735036 |
| AlexaFluor700 anti-mouse CD45 (clone 30-F11) | Invitrogen | Cat# 56-0451-82; RRID:AB_891454 |
| APC anti-mouse CD44 (clone IM7) | Thermo Fisher Scientific | Cat# 17-0441-82; RRID: AB_469390 |
| APC anti-mouse MHCII (clone M5/114.15.2) | Thermo Fisher Scientific | Cat# 17-5321-82; RRID: AB_469455 |
| APC anti-mouse CD4 (clone GK1.5) | Thermo Fisher Scientific | Cat# 47-0041-82; RRID:AB_11218896 |
| APC anti-mouse CD103 (clone 2E7) | Thermo Fisher Scientific | Cat# 17-1031-82; RRID: AB_1106992 |
| e450 anti-mouse MHCII (clone M5/114.15.2) | Thermo Fisher Scientific | Cat#48-5321-82; RRID: AB_1272204 |
| e450 anti-mouse Foxp3 (clone FJK-16S) | Thermo Fisher Scientific | Cat#48-5773-82; RRID: AB_1518812 |
| BV480 anti-mouse CD44 (clone IM7) | BD Biosciences | Cat# 566116; RRID:AB_655116 |
| BV605 anti-mouse Ly6C (clone HK1.4) | Biolegend | Cat#128035; RRID: RRID:AB_2562352 |
| BV605 anti-mouse CD8 (clone 53-6.7) | Biolegend | Cat# 100744; RRID: AB_2562609 |
| BV605 anti-mouse TCR-beta (clone H57-597) | BD Biosciences | Cat# 562840; RRID: AB_2687544 |
| BV650 anti-mouse CD11c (clone N418) | Biolegend | Cat# 117339; RRID:AB_2562414 |
| BV650 anti-mouse CD4 (clone RM4-5) | BD Biosciences | Cat#563747; RRID: AB_2716859 |
| BV711 anti-mouse CD11b (clone M1/70) | Biolegend | Cat#101241; RRID:AB_11218791 |
| BV711 anti-mouse CD3 (clone 145-2C11) | BD Biosciences | Cat#563123; RRID: AB_2687954 |
| BV786 anti-mouse CD64 (clone X54-5/7.1) | BD Biosciences | Cat#741024; RRID:AB_2740644 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Collagenase from Clostridiumhistolyticum, Type XI | Sigma-Aldrich | Cat#C9407 |
| Collagenase from Clostridiumhistolyticum, Type I | Sigma-Aldrich | Cat#SCR103 |
| Collagenase from Clostridiumhistolyticum, Type IV | Sigma-Aldrich | Cat#lS00418 |
| DNase | Sigma-Aldrich | Cat#DN25 |
| Hyaluronidase from bovine testes | Sigma-Aldrich | Cat#H3506 |
| α-hemolysin from Staphylococcus aureus | Sigma-Aldrich | Cat#H9395 |
| Tryptic Soy Medium | BD-Bacto | Cat#211825 |
| Brain Heart Infusion Medium | BD-Bacto | Cat#221570 |
| Recombinant Murine IL-1β | Peprotech | Cat#211-11B |
| Recombinant Murine IL-1α | Peprotech | Cat#211-11A |
| PE-conjugated 2w-loaded I-A(b) Tetramer | Provided by James Moon | N/A |
| Fetal bovine serum | Fisher scientific | Cat#SH3054103 |
| Fetal calf serum | Fisher scientific | Cat#SH3007303 |
| mGM-CSF | PeproTech | Cat#315-03 |
| Critical Commercial Assays | ||
| Pierce LDH cytotoxicity assay kit | Thermo Scientific | Cat#88953 |
| IL1β ELISA kit | R&D systems | Cat#DY401-05 |
| RNeasy Fibrous Tissue Mini Kit | Qiagen | Cat#74704 |
| gentleMACS M Tubes | Miltenyi Biotec | Cat#130-093-236 |
| RNAlater Stabilization Solution | Life Technologies | Cat#AM7020 |
| EasySep™ Mouse PE Positive Selection Kit | StemCell Technologies | Cat# 18554 |
| Mouse FoxP3 Buffer Set | eBiosciences | Cat# 00-5523-00 |
| Ghost Dye™ Violet 510 Live/Dead Stain | Tonbo Biosciences | Cat# 13-0870-T100 |
| Ghost Dye Violet 780 Live/Dead Stain | Tonbo | Cat# 13-0865-T100 |
| Experimental Models: Organisms/Strains | ||
| SPF B6 mice | Jackson Laboratory | C57BL/6J Cat#000664 |
| Ccr7−/− | Jackson Laboratory | B6.129P2(C)-Ccr7tm1Rfor/J Cat#006621 |
| Il1r1−/− | Jackson Laboratory | B6.129S7-Il1r1tm1Imx/J Cat#003245 |
| Experimental Models: Organisms/Strains | ||
| Staphylococcus epidermidis Tü3298 | Provided by Michael Otto (Augustin and Gotz, 1990) | N/A |
| Staphylococcus aureus SF8300 parent strain | Provided by Binh Diep (Diep et al., 2008b) | N/A |
| Staphylococcus aureus SF8300 lacking ermB-encoding plasmid | Provided by Binh Diep | N/A |
| Staphylococcus aureus SF8300 Δhla | Provided by Binh Diep (Diep et al., 2016) | N/A |
| Staphylococcus aureus SF8300 Δpsm | Generated for these studies | N/A |
| Staphylococcus aureus USA300 NRS384 | NARSA | N/A |
| Staphylococcus aureus AD04.E17 | Provided by Heidi Kong & Julia Segre | N/A |
| Staphylococcus epidermidis NIHLM015- 2D12 | Provided by Heidi Kong & Julia Segre | N/A |
| Staphylococcus epidermidis NIHLM037- 5A02 | Provided by Heidi Kong & Julia Segre | N/A |
| Staphylococcus aureus Mu50φ | Provided by Paul Sullam | N/A |
| Staphylococcus epidermidis BCM060 | BEI | N/A |
| Staphylococcus epidermidis W23144 | BEI | Cat#HM-142 |
| Staphylococcus epidermidis SK135 | BEI | Cat#HM-118 |
| Staphylococcus hominis SK119 | BEI | Cat#HM-119 |
| Corynebacterium accolens ATCC 49725 | ATCC | N/A |
| Corynebacterium propinquum DSM44285 | Provider by Katherine Lemon | N/A |