Table-3.

Oligonucleotide primers for IBV, NDV, AIV, MG, and MS.

Pathogen	Primer name		Sequence	Annealing temperature (°C)	References
IBV	N primer430 bp	F Primer (N+) R Primer (N−)	5`-GAAGAAAACCAGTCCCAGATGCTTGG-3` 5`-GTTGGAATAGTGCGCTTGCAATACCG-3`	60	[35]
NDV	Primer F (APMV1-F) 535 bp	(APMV1-F-F) (APMV1-F-R)	5`-ATGGGCYCCAGACYCTTCTAC-3` 5`-CCTGAGGAGAGGCATTTGCTA-3`	60	[33]
AIV	AIV M2 detection 244 bp	M52C-M2-F 253R-M2-R	5/- CTTCTAACCGAGGTCGAAACG -3/ 5/- AGGGCATTTTGGACAAAGCGTCTA -3/	52	[34]
	AIV H9 typing 549 bp	H9-For H9-Rev	5/-ATT CAA GAC GCC CAA TAC AC-3/ 5/-TGA CCA ACC TCC CTC TAT GA-3/	52	[36]
	AIV N2 typing 1420 bp	AIVN2-F AIVN2-F	5/- GTAAAAATGAATCCAAATCAAAAG-3/ 5/- GTAAAAATGAATCCAAATCAAAAG-3/	52	[32]
MG	(mgC2) 237 bp	(F primer) (R primer)	5`-CGC AAT TTG GTC CTA ATC CCC AAC A -3` 5`-TAA ACC CAC CTC CAG CTT TAT TTC C-3`	72	[38]
MS	16S rRNA 214 bp	F primer R primer	5`-GAG AAG CAA AAT AGT GAT ATC A-3` 5`- CAG TGG TCT CCG AAG TTA ACA A-3`	72	[39]

NDV=Newcastle disease virus, IBV=Infectious bronchitis virus, MG=Mycoplasma gallisepticum, MS=Mycoplasma synoviae, AIV=Avian influenza virus